NCC

Mfr.No.	SPC-402, SPC-402D, SPC-402D-A390, SPC-402D-A488, SPC-402D-A594, SPC-402D-APC, SPC-402D-BI, SPC-402D-FITC, SPC-402D-HRP, SPC-402D-PCP, SPC-402D-RPE, SPC-402S
Immunogen Species	Rat
Alternative Names	SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	1 ug/ml of SPC-402 was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Rat NCC Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal, Rattus Norvegicus (Rat)
Immunogen Region	Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
Launch Date	2010/06/11
Net Weight (g)	0.1
Reactivity	Dog, Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Rattus Norvegicus (Rat)
Species Reactivity Medium	Mouse, Rat, Dog, Human

Antigen	NCC
Swissprot	P55018, P55017
Accession Number	NP_062218, NP_000330
Gene Id	54300, 6559
Target	NCC
Research Area	Cancer, Pumps/Transporters, Ion Pumps/Transporters, Neuroscience

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Purification	Affinity Purified
Scientific Background	NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~160kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IEM, IHC, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF ; IEM
Dilution	WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11907786-1	SPC-402D	Unconjugated	100 ug	$348	Add to Cart
BHA11907786-2	SPC-402D-A390	ATTO 390	100 ug	$403	Add to Cart
BHA11907786-3	SPC-402D-A488	ATTO 488	100 ug	$402	Add to Cart
BHA11907786-4	SPC-402D-A594	ATTO 594	100 ug	$402	Add to Cart
BHA11907786-5	SPC-402D-APC	APC	100 ug	$401	Add to Cart
BHA11907786-6	SPC-402D-BI	Biotin	100 ug	$397	Add to Cart
BHA11907786-7	SPC-402D-FITC	FITC	100 ug	$393	Add to Cart
BHA11907786-8	SPC-402D-HRP	HRP	100 ug	$389	Add to Cart
BHA11907786-9	SPC-402D-PCP	PerCP	100 ug	$401	Add to Cart
BHA11907786-10	SPC-402D-RPE	RPE	100 ug	$399	Add to Cart
BHA11907786-11	SPC-402S	Unconjugated	12 ug	$45	Add to Cart

Rabbit Anti-Rat NCC Polyclonal (SPC-402)