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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-Methylated Lysine Polyclonal (SPC-158)

BHA11906828
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Email
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Antigen Methylated Lysine
Target Methylated Lysine
Research Area Cell Signaling, Post-translational Modifications, Methylation
Buffer PBS, 50% glycerol
Concentration 1 mg/ml
Dilutions WB (1:20000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nepsilon-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
Shipping Temperature Blue Ice or 4°C
Specificity Detects proteins containing methylated lysine residues.
Storage -20ºC
Storage Buffer PBS, 50% glycerol *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IP, WB, ICC/IF
Description WB ; ICC ; IF ; IP ; ELISA
Dilution WB (1:20000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Yang X.J. (2005) Oncogene. 24:1653-1662.

2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308.

3. Yang X.J. (2004) Bioessays 26:1076-1087.

4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143.

5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218.

6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674.

7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906828-1 SPC-158D Unconjugated 100 ug $348
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BHA11906828-2 SPC-158D-A390 ATTO 390 100 ug $403
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BHA11906828-3 SPC-158D-A488 ATTO 488 100 ug $402
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BHA11906828-4 SPC-158D-A594 ATTO 594 100 ug $402
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BHA11906828-5 SPC-158D-APC APC 100 ug $401
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BHA11906828-6 SPC-159F Biotin 400 ul $395
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BHA11906828-7 SPC-158D-FITC FITC 100 ug $393
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BHA11906828-8 SPC-160F HRP 400 ul $413
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BHA11906828-9 SPC-158D-PCP PerCP 100 ug $401
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BHA11906828-10 SPC-158D-RPE RPE 100 ug $399
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BHA11906828-11 SPC-158S Unconjugated 12 ug $45
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