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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-Bovine Ubiquitin Polyclonal (SPC-119)

BHA11906543
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Antigen Ubiquitin
Swissprot P0CG53
Accession Number NP_776558.1
Gene Id 281370
Target Ubiquitin
Research Area Cell Signaling, Post-translational Modifications, Ubiquitination, Neurodegeneration, Alzheimer's Disease, Neuroscience
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkappaBalpha and p27 (6).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~10kDa. It also recognizes ubiquinated proteins.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189.

2. Smalle J., Vierstra R.D. (2004) Anu Rev Plant Biol. 55: 555-590.

3. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57.

4. Boston Biochem: "Ubiquitin Proteasome Pathway Overview” http://www.bostonbiochem.com/upp.php

5. Yang M., et al. (1998) J Exp Med. 187: 1835-1846.

6. Chen Z.J., et al. (1996) Cell 84: 853-862.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906543-1 SPC-119B Unconjugated 200 ul $344
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BHA11906543-2 SPC-119B-A390 ATTO 390 200 ul $448
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BHA11906543-3 SPC-119B-A488 ATTO 488 200 ul $445
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BHA11906543-4 SPC-119B-A594 ATTO 594 200 ul $445
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BHA11906543-5 SPC-119B-APC APC 200 ul $444
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BHA11906543-6 SPC-119B-BI Biotin 200 ul $435
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BHA11906543-7 SPC-119B-FITC FITC 200 ul $425
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BHA11906543-8 SPC-119B-HRP HRP 200 ul $418
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BHA11906543-9 SPC-119B-PCP PerCP 200 ul $444
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BHA11906543-10 SPC-119B-RPE RPE 200 ul $437
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BHA11906543-11 SPC-119S Unconjugated 12 ul $45
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