Phospho-PI3K p85 alpha (Tyr607) Antibody

Mfr.No.	AF3241
Alternative Names	GRB 1 ; GRB1 ; p85 alpha ; p85 ; P85A_HUMAN ; Phosphatidylinositol 3 kinase associated p 85 alpha ; Phosphatidylinositol 3 kinase regulatory 1 ; Phosphatidylinositol 3 kinase regulatory subunit alpha ; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 (p85 alpha) ; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha ; Phosphatidylinositol 3-kinase regulatory subunit alpha ; Phosphoinositide 3 kinase regulatory subunit 1 (alpha) ; Phosphoinositide 3 kinase regulatory subunit 1 (p85 alpha) ; Phosphoinositide 3 kinase regulatory subunit 1 ; Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha) ; PI3 kinase p85 subunit alpha ; PI3-kinase regulatory subunit alpha ; PI3-kinase subunit p85-alpha ; PI3K ; PI3K regulatory subunit alpha ; Pik3r1 ; PtdIns 3 kinase p85 alpha ; PtdIns-3-kinase regulatory subunit alpha ; PtdIns-3-kinase regulatory subunit p85-alpha ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	PIK3R1 is a regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alteatively spliced isoforms have been described.
Immunogen	A synthesized peptide derived from human PI3-kinase p85- alpha around the phosphorylation site of Tyrosine 607.
Molecular Weight	Observed Mol.Wt.: 80kDa. Predicted Mol.Wt.: 84kDa.
Reactivity	Human, Mouse, Pig, Rat
Specificity	Phospho-PI3-kinase p85- alpha (Tyr607) Antibody detects endogenous levels of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.
Subcellular Location	Cytoplasmic

Uniprot

P27986

Gene Id

5295

Sequence

10	20	30	40	50
MSAEGYQYRA	LYDYKKEREE	DIDLHLGDIL	TVNKGSLVAL	GFSDGQEARP
60	70	80	90	100
EEIGWLNGYN	ETTGERGDFP	GTYVEYIGRK	KISPPTPKPR	PPRPLPVAPG
110	120	130	140	150
SSKTEADVEQ	QALTLPDLAE	QFAPPDIAPP	LLIKLVEAIE	KKGLECSTLY
160	170	180	190	200
RTQSSSNLAE	LRQLLDCDTP	SVDLEMIDVH	VLADAFKRYL	LDLPNPVIPA
210	220	230	240	250
AVYSEMISLA	PEVQSSEEYI	QLLKKLIRSP	SIPHQYWLTL	QYLLKHFFKL
260	270	280	290	300
SQTSSKNLLN	ARVLSEIFSP	MLFRFSAASS	DNTENLIKVI	EILISTEWNE
310	320	330	340	350
RQPAPALPPK	PPKPTTVANN	GMNNNMSLQD	AEWYWGDISR	EEVNEKLRDT
360	370	380	390	400
ADGTFLVRDA	STKMHGDYTL	TLRKGGNNKL	IKIFHRDGKY	GFSDPLTFSS
410	420	430	440	450
VVELINHYRN	ESLAQYNPKL	DVKLLYPVSK	YQQDQVVKED	NIEAVGKKLH
460	470	480	490	500
EYNTQFQEKS	REYDRLYEEY	TRTSQEIQMK	RTAIEAFNET	IKIFEEQCQT
510	520	530	540	550
QERYSKEYIE	KFKREGNEKE	IQRIMHNYDK	LKSRISEIID	SRRRLEEDLK
560	570	580	590	600
KQAAEYREID	KRMNSIKPDL	IQLRKTRDQY	LMWLTQKGVR	QKKLNEWLGN
610	620	630	640	650
ENTEDQYSLV	EDDEDLPHHD	EKTWNVGSSN	RNKAENLLRG	KRDGTFLVRE
660	670	680	690	700
SSKQGCYACS	VVVDGEVKHC	VINKTATGYG	FAEPYNLYSS	LKELVLHYQH
710	720
TSLVQHNDSL	NVTLAYPVYA	QQRR

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.