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Home / Primary Antibodies / Monoclonal Antibodies

Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody

SKU : BHA21300567

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Mfr.No.	ALM-025-B, ALM-025-B_50 mcg
Lead Time	1-2 Business Days
Immunogen Species	Human
Background	Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.
Clonality	Monoclonal
Description	Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only. Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody has been tested in western blot and direct flow cytometry applications and is specially suited to experiments requiring simultaneous labeling of different markers.
Formulation	PBS pH7.4, 1% BSA with 0.05% sodium azide
Host	Mouse
Immunogen Region	Extracellular
Isotype	IgM
Reactivity	Human
Reconstitution	50 ul double distilled water (DDW).
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Synonyms	TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
UNSPSC Code	41116161
Note	Homology: Recognizes Orai1 from human samples only Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry Specifictiy: ORAI1 AB Specifictiy: Recognizes only human Orai1. Is Toxin: No Standard quality control of each lot: Western blot analysis (unlabeled antibody, #ALM-025), and direct flow cytometry (labeled antibody).

Accession Number	Q63633
Gene Id	84876
Target	TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
Clone No	3F11, D10, B9
Sequence	Synthetic peptide mapping to an extracellular sequence of human Orai1

Conjugates	Biotin.

Concentration	1 mg/ml
Form	Lyophilized powder
Preservative	1% BSA, 0.05% NaN3
Purity	Affinity purified on immobilized antigen.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Applications	FC, WB
Dilution	Contact Alomone for information

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA21300567-1	ALM-025-B_50 mcg	50 mcg	$797		Add to Cart

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