Mouse Anti-Mouse MDC1 Monoclonal IgG1

Mfr.No.	SMC-197, SMC-197D, SMC-197D-A390, SMC-197D-A488, SMC-197D-A594, SMC-197D-APC, SMC-197D-BI, SMC-197D-FITC, SMC-197D-HRP, SMC-197D-PCP, SMC-197D-RPE, SMC-197S
Immunogen Species	Mouse
Alternative Names	Nuclear factor with BRCT domains1 Antibody, mediator of DNA damage checkpoint 1 Antibody
Categories	Antibodies
Cellular Localization	Nucleus
Certificate Of Analysis	0.5 ug/ml of SMC-197 was sufficient for detection of MDC1 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.
Clonality	Monoclonal
Description	Mouse Anti-Mouse MDC1 Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus, Mus Musculus (Mouse)
Immunogen Region	GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Isotype	IgG1
Launch Date	2011/08/30
Net Weight (g)	0.1
Reactivity	Mouse, Human, Bovine, Chimpanzee
Recombinant	FALSE
Regulatory	RUO
Species	Mus Musculus (Mouse)
Species Reactivity Medium	Mouse, Bovine, Chimpanzee, Human

Antigen	MDC1
Swissprot	Q5PSV9
Accession Number	NP_001010833.2
Gene Id	240087
Target	MDC1
Clone No	P2B11
Research Area	Cancer, Oxidative Stress, Cell Signaling, Epigenetics and Nuclear Signaling

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901529-1	SMC-197D	Unconjugated	100 ug	$312	Add to Cart
BHA11901529-2	SMC-197D-A390	ATTO 390	100 ug	$367	Add to Cart
BHA11901529-3	SMC-197D-A488	ATTO 488	100 ug	$366	Add to Cart
BHA11901529-4	SMC-197D-A594	ATTO 594	100 ug	$366	Add to Cart
BHA11901529-5	SMC-197D-APC	APC	100 ug	$365	Add to Cart
BHA11901529-6	SMC-197D-BI	Biotin	100 ug	$361	Add to Cart
BHA11901529-7	SMC-197D-FITC	FITC	100 ug	$357	Add to Cart
BHA11901529-8	SMC-197D-HRP	HRP	100 ug	$352	Add to Cart
BHA11901529-9	SMC-197D-PCP	PerCP	100 ug	$365	Add to Cart
BHA11901529-10	SMC-197D-RPE	RPE	100 ug	$362	Add to Cart
BHA11901529-11	SMC-197S	Unconjugated	12 ug	$45	Add to Cart

MDC1 Antibody, Clone P2B11 (SMC-197)