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Home / Primary Antibodies / Monoclonal Antibodies

HSP60 Antibody, Clone LK2 (SMC-111)

BHA11900161
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Email
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Antigen HSP60
Swissprot P10809
Accession Number NP_002147.2
Gene Id 3329
Target HSP60
Clone No LK2
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tags and Cell Markers, Organelle Markers, Protein Trafficking
Buffer PBS, 50% glycerol, 0.1mM PMSF
Concentration 1 mg/ml
Dilutions WB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts.
It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~60kDa.
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, WB, FCM
Description WB ; IHC ; FCM
Dilution WB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Hartl, F.U. (1996) Nature 381: 571-579.

2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366.

3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858.

4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283.

5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71.

6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938.

7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58.

8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128.

9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.

10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556.

11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929.

12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.

Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900161-1 SMC-111B Unconjugated 200 ug $310
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BHA11900161-2 SMC-111B-A390 ATTO 390 200 ug $414
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BHA11900161-3 SMC-111B-A488 ATTO 488 200 ug $411
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BHA11900161-4 SMC-111B-A594 ATTO 594 200 ug $411
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BHA11900161-5 SMC-111B-APC APC 200 ug $410
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BHA11900161-6 SMC-111B-BI Biotin 200 ug $401
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BHA11900161-7 SMC-111B-FITC FITC 200 ug $393
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BHA11900161-8 SMC-111B-HRP HRP 200 ug $386
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BHA11900161-9 SMC-111B-PCP PerCP 200 ug $410
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BHA11900161-10 SMC-111B-RPE RPE 200 ug $403
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BHA11900161-11 SMC-111S Unconjugated 12 ug $45
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