Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa

Mfr.No.	SMC-150, SMC-150D, SMC-150D-A390, SMC-150D-A488, SMC-150D-A594, SMC-150D-APC, SMC-150D-BI, SMC-150D-FITC, SMC-150D-HRP, SMC-150D-PCP, SMC-150D-RPE, SMC-150S
Immunogen Species	Yeast
Alternative Names	DNAJA2 Antibody, CPR3 Antibody, HIRIP4 Antibody, DNAJ Antibody, DNJ3 Antibody, DJ3 Antibody, RDJ2 Antibody, HIRa interacting protein4 Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus
Certificate Of Analysis	0.5 ug/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Full length protein yeast HSP40 (YDJ1), Saccharomyces Cerivisiae (Yeast)
Immunogen Region	Full length protein yeast HSP40 (YDJ1)
Isotype	IgG1 Kappa
Launch Date	2008/11/01
Net Weight (g)	0.1
Reactivity	Yeast
Recombinant	FALSE
Regulatory	RUO
Species	Saccharomyces Cerivisiae (Yeast)
Species Reactivity Medium	Yeast

Antigen	HSP40
Swissprot	P25491
Accession Number	NP_014335.1
Gene Id	855661
Target	HSP40
Clone No	1G10.H8
Research Area	Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking

Buffer	50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:2000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~40kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.
Storage	-20ºC
Storage Buffer	50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IP, WB
Description	WB ; IP ; ELISA
Dilution	WB (1:2000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 2836. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309316. 3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830. 5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 28742878. 6. Cyr D.M., et al. (1992) J Biol Chem. 267: 2092720931. 7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885890.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11900700-1	SMC-150D	Unconjugated	100 ug	$329	Add to Cart
BHA11900700-2	SMC-150D-A390	ATTO 390	100 ug	$385	Add to Cart
BHA11900700-3	SMC-150D-A488	ATTO 488	100 ug	$384	Add to Cart
BHA11900700-4	SMC-150D-A594	ATTO 594	100 ug	$384	Add to Cart
BHA11900700-5	SMC-150D-APC	APC	100 ug	$383	Add to Cart
BHA11900700-6	SMC-150D-BI	Biotin	100 ug	$379	Add to Cart
BHA11900700-7	SMC-150D-FITC	FITC	100 ug	$374	Add to Cart
BHA11900700-8	SMC-150D-HRP	HRP	100 ug	$369	Add to Cart
BHA11900700-9	SMC-150D-PCP	PerCP	100 ug	$383	Add to Cart
BHA11900700-10	SMC-150D-RPE	RPE	100 ug	$380	Add to Cart
BHA11900700-11	SMC-150S	Unconjugated	12 ug	$45	Add to Cart

HSP40, YDJ1 Antibody, Clone 1G10.H8 (SMC-150)