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Home / Primary Antibodies / Monoclonal Antibodies

HSP27 Antibody, Clone 8A7 (SMC-114)

BHA11900221
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Email
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Antigen HSP27
Swissprot P04792 
Accession Number NP_001532.1
Gene Id 3315
Target HSP27
Clone No 8A7
Research Area Cell Signaling, Chaperone Proteins, Microfilaments, Actin, Actin Assembly, Microtubules, Cancer, Tumor Biomarkers, Cardiovascular System, Atherosclerosis, Heart, Contractility, Heat Shock, Protein Trafficking, Cytoskeleton
Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction.
Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications FACS, IHC, IP, WB, ICC/IF, FCM
Description WB ; IHC ; ICC ; IF ; IP ; FCM ; FACS
Dilution WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Welch W.J. (1985) J Biol. Chem. 260: 3058-3062.

2. Kim K.K., Kim R., and Kim S. (1998) Nature 394(6693): 595-599.

3. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56.

4. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229.

5. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70.

6. Welsh M.J., Wu W., Parvinem M., and Gilmont R.R. (1996) Biol. Of Reprod. 55: 141-151.

7. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26.

8. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.

9. Jia, Y. et al. (2001) J. Biol. Chem. 276(43):39911-39918.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900221-1 SMC-114D Unconjugated 100 ug $285
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BHA11900221-2 SMC-114D-A390 ATTO 390 100 ug $340
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BHA11900221-3 SMC-114D-A488 ATTO 488 100 ug $339
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BHA11900221-4 SMC-114D-A594 ATTO 594 100 ug $339
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BHA11900221-5 SMC-114D-APC APC 100 ug $338
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BHA11900221-6 SMC-114D-BI Biotin 100 ug $335
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BHA11900221-7 SMC-114D-FITC FITC 100 ug $329
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BHA11900221-8 SMC-114D-HRP HRP 100 ug $325
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BHA11900221-9 SMC-114D-PCP PerCP 100 ug $338
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BHA11900221-10 SMC-114D-RPE RPE 100 ug $336
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BHA11900221-11 SMC-114S Unconjugated 12 ug $45
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