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Home / Primary Antibodies / Monoclonal Antibodies

FKBP52 Antibody, Clone Hi52C (SMC-139)

BHA11900560
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Email
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Antigen FKBP52
Swissprot Q02790
Accession Number NP_002005.1
Gene Id 2288
Target FKBP52
Clone No Hi52C
Research Area Cancer, Heat Shock, Cell Signaling, Nuclear Import/Export, Protein Trafficking
Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3).
The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3).
FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5ug); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394.

2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600.

3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353.

4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7):3194-3201.

5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900560-1 SMC-139D Unconjugated 100 ug $352
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BHA11900560-2 SMC-139D-A390 ATTO 390 100 ug $407
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BHA11900560-3 SMC-139D-A488 ATTO 488 100 ug $406
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BHA11900560-4 SMC-139D-A594 ATTO 594 100 ug $406
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BHA11900560-5 SMC-139D-APC APC 100 ug $405
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BHA11900560-6 SMC-139D-BI Biotin 100 ug $402
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BHA11900560-7 SMC-139D-FITC FITC 100 ug $397
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BHA11900560-8 SMC-139D-HRP HRP 100 ug $393
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BHA11900560-9 SMC-139D-PCP PerCP 100 ug $405
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BHA11900560-10 SMC-139D-RPE RPE 100 ug $403
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BHA11900560-11 SMC-139S Unconjugated 12 ug $45
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