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Home / Primary Antibodies / Monoclonal Antibodies

ERp57 (GRP58) Antibody, Clone 4F9 (SMC-266)

BHA11902367
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Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
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Antigen ERp57
Swissprot P30101
Accession Number NP_005304.3
Gene Id 2923
Target ERp57
Clone No 4F9
Research Area Cell Signaling, ER Proteins, Protein Trafficking
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 1 mg/ml
Dilutions WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~57 kDa protein.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications WB, ICC/IF
Description WB ; ICC ; IF
Dilution WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.

2. Williams D.B. (2005) J Cell Sci. 119: 615-623

3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.

4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.

5. Oliver J.D., et al. (1997) Science 275: 86-88.

6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.

7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.

8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.

9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11902367-1 SMC-266D Unconjugated 100 ug $337
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BHA11902367-2 SMC-266D-A390 ATTO 390 100 ug $392
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BHA11902367-3 SMC-266D-A488 ATTO 488 100 ug $391
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BHA11902367-4 SMC-266D-A594 ATTO 594 100 ug $391
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BHA11902367-5 SMC-266D-APC APC 100 ug $390
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BHA11902367-6 SMC-266D-BI Biotin 100 ug $386
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BHA11902367-7 SMC-266D-FITC FITC 100 ug $381
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BHA11902367-8 SMC-266D-HRP HRP 100 ug $377
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BHA11902367-9 SMC-266D-PCP PerCP 100 ug $390
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BHA11902367-10 SMC-266D-RPE RPE 100 ug $388
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BHA11902367-11 SMC-266S Unconjugated 12 ug $45
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