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Home / Primary Antibodies / Monoclonal Antibodies

Dityrosine Antibody, Clone 10A6 (SMC-521)

BHA11905692
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Email
orders@biohippo.com
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Antigen Dityrosine
Target Dityrosine
Clone No 10A6
Research Area Cancer, Oxidative Stress, Protein Oxidation, Cell Signaling, Post-translational Modifications, Oxidation
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 1 mg/ml
Dilutions WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
Shipping Temperature Blue Ice or 4°C
Specificity Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Trademark MOLECULAR SIGNATURE
Applications ELISA, FACS, WB, ICC/IF, FCM
Description WB ; ICC ; IF ; FACS ; FCM ; ELISA
Dilution WB (1:1000); ICC/IF (1:50); FACS (1:50); FCM (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25(3-4):233-247.

2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11905692-1 SMC-521D Unconjugated 100 ug $417
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BHA11905692-2 SMC-521D-A390 ATTO 390 100 ug $472
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BHA11905692-3 SMC-521D-A488 ATTO 488 100 ug $471
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BHA11905692-4 SMC-521D-A594 ATTO 594 100 ug $471
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BHA11905692-5 SMC-521D-APC APC 100 ug $470
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BHA11905692-6 SMC-521D-BI Biotin 100 ug $467
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BHA11905692-7 SMC-521D-FITC FITC 100 ug $462
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BHA11905692-8 SMC-521D-HRP HRP 100 ug $458
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BHA11905692-9 SMC-521D-PCP PerCP 100 ug $470
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BHA11905692-10 SMC-521D-RPE RPE 100 ug $468
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BHA11905692-11 SMC-521S Unconjugated 12 ug $45
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