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Home / Primary Antibodies

CD45 Antibody (Leukocyte marker) (V3223)

SKU : BHA17113613

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NSJ Bioreagents

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Mfr.No.	V3223, V3223-20UG, V3223-100UG
Clonality	Monoclonal
Description	CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	Human T-lymphocytes were used as the immunogen for the CD45 antibody.
Isotype	IgG2a,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cell surface and cytoplasmic

Antigen	CD45
Uniprot	P08575
Clone No	F10-89-4

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	CD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Applications	FACS, IF, IHC-P
Dilution	Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17113613-1	V3223-20UG	20ug	$219		Add to Cart
BHA17113613-2	V3223-100UG	100ug	$499		Add to Cart

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