Anti-V5 is a mouse monoclonal antibody, affinity purified from culture supernatant of hybridoma cells grown in a bioreactor.
Isotype
IgG1
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: Anti-V5 Antibody detects (GKPIPNPLLGLDST) tag fusion protein.
Format
Liquid
Purity
Protein A affinity chromatography
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IF, IHC, ICC, ELISA
Description
Western blot analysis: 1:1000 ELISA: 1:5000-10,000 IHC: 1:50-1:100. Other applications will need optimization from end users., WB ; IF ; IHC ; ICC ; ELISA
Dilution
WB
1:1000
ELISA
1:5000-10,000
IHC
1:50-1:100
Reference
Al-Maqtari, AT et al. "Promoting differentiation and survival of human ckit+cardiac progenitor cells ex vivo.." Phd Dissertation (University of Louisville) : (2015). DOI: http://ir.library.louisville.edu/etd/2228/.
Al-Maqtari, T et al. "Transcription factor-induced activation of cardiac gene expression in human c-kit+ cardiac progenitor cells." PLoS One 3:e0174242 (2017). DOI: 10.1371/journal.pone.0174242. PubMed: 28355297. Application: Western Blot.
Gueller, S et al. "Adaptor protein Lnk associates with Tyr568 in c-Kit." Biochem J 413 (2):241-245 (2008). DOI: 10.1042/BJ20080102. Application: Western Blot.
Gueller, S et al. "Adaptor protein Lnk binds to PDGF receptor and inhibits PDGF-dependent signaling." Exp Hematol. 39 (5):591-600 (2011). DOI: 10.1016/j.exphem.2011.02.00. Application: Western Blot.
Gueller, S et al. "Adaptor protein Lnk inhibits c-Fms-mediated macrophage function." J Leukoc Biol 88 (4):699-706 (2010). DOI: 0.1189/jlb.0309185. Application: Western Blot.
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