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Home / Primary Antibodies / Polyclonal Antibodies

Anti-USP16 Antibody Picoband®

SKU : BHA21001818

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Mfr.No.	A05795, A05795-10ug, A05795-Biotin, A05795-Cy3, A05795-Dylight488, A05795-Dylight550, A05795-Dylight594, A05795-FITC, A05795-HRP, A05795-APC, A05795-PE, A05795-iFluor647, A05795-carrier-free
Alternative Names	ubiquitin specific peptidase 16
Background	Ubiquitin carboxyl-terminal hydrolase 16 is an enzyme that in humans is encoded by the USP16 gene. It is mapped to 21q21.3. This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alteate transcriptional splice variants, encoding different isoforms, have been characterized.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-USP16 Antibody Picoband® catalog # A05795. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of h
Host	Rabbit
Immunogen Region	E.coli-derived human USP16 recombinant protein (Position: K9-L823).
Isotype	Rabbit IgG
Molecular Weight	100 kDa
Reactivity	Human, Monkey, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Ubiquitin carboxyl-terminal hydrolase 16; Deubiquitinating enzyme 16; Ubiquitin thioesterase 16; Ubiquitin-processing protease UBP-M; Ubiquitin-specific-processing protease 16; USP16; MSTP039
Tissue Specificity	Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	Q9Y5T5
Gene Id	10600
Gene Name	USP16
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5ug/ml, Human, Monkey<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Adoo, M., Sikandar, S., Mitra, S. S., Kuo, A., Nicolas Di Robilant, B., Haro-Acosta, V., Ouadah, Y., Quarta, M., Rodriguez, J., Qian, D., Reddy, V. M., Cheshier, S., Gaer, C. C., Clarke, M. F. Usp16 contributes to somatic stem-cell defects in Down's syndrome. Nature 501: 380-384, 2013.<br /><br />2. Cai, S.-Y., Babbitt, R. W., Marchesi, V. T. A mutant deubiquitinating enzyme (Ubp-M) associates with mitotic chromosomes and blocks cell division. Proc. Nat. Acad. Sci. 96: 2828-2833, 1999.<br /><br />3. Joo, H.-Y., Zhai, L., Yang, C., Nie, S., Erdjument-Bromage, H., Tempst, P., Chang, C., Wang, H. Regulation of cell cycle progression and gene expression by H2A deubiquitination. Nature 449: 1068-1072, 2007.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001818-1	A05795	100 ug	-	$370		Add to Cart
BHA21001818-2	A05795-10ug	10 ug	Sample	$99		Add to Cart
BHA21001818-3	A05795-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001818-4	A05795-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001818-5	A05795-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001818-6	A05795-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001818-7	A05795-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001818-8	A05795-FITC	100 ug	FITC	$570		Add to Cart
BHA21001818-9	A05795-HRP	100 ug	HRP	$570		Add to Cart
BHA21001818-10	A05795-APC	100 ug	APC	$670		Add to Cart
BHA21001818-11	A05795-PE	100 ug	PE	$670		Add to Cart
BHA21001818-12	A05795-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001818-13	A05795-carrier-free	100 ug	carrier-free	$370		Add to Cart

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