Calnexin ; CANX ; CNX ; IP90 ; Major histocompatibility complex class I antigen-binding protein p88 ; P90
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
5q35.3
Clonality
monoclonal
Description
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Host
Mouse
Immunogen
Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
Isotype
IgG2b Kappa
Positive Control
HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P27824
Gene Id
821
Clone No
CANX, 1541
Symbol
CANX
Weight
90kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
ELISA, WB, IHC-F
Description
Order Ab without BSA, Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., ELISA ; WB ; IHC-F
Dilution
ELISA
Use Ab at 2-4μg/ml for coating
Western Blotting
1-2μg/ml
Immunohistology (Formalin-fixed)
1-2μg/ml for 30 minutes at RT
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Reviews of Anti-undefined Antibody CANX,1541 Unconjugated BHA10208088