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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Topoisomerase II alpha/TOP2A Antibody Picoband®

SKU : BHA21002372

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Mfr.No.	A00953, A00953-10ug, A00953-Biotin, A00953-Cy3, A00953-Dylight488, A00953-Dylight550, A00953-Dylight594, A00953-FITC, A00953-HRP, A00953-APC, A00953-PE, A00953-iFluor647, A00953-carrier-free
Alternative Names	DNA topoisomerase II alpha
Background	The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract1. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Topoisomerase II alpha/TOP2A Antibody Picoband® catalog # A00953. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regula
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence in the middle region of human Topoisomerase II alpha/TOP2A, identical to the related mouse and rat sequences.
Isotype	Rabbit IgG
Molecular Weight	174 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2A; TOP2
Tissue Specificity	Liver.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P11388
Gene Id	7153
Gene Name	TOP2A
Subcellular Location	Lysosome membrane; Single-pass type I membrane protein.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.25-0.5ug/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 2ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Miller, K. G.; Liu, L. F.; Englund, P. T. : A homogeneous type II DNA topoisomerase from HeLa cell nuclei. J. Biol. Chem. 256: 9334-9339, 1981. <br /><br />2. Mondal, N.; Parvin, J. D. : DNA topoisomerase II-alpha is required for RNA polymerase II transcription on chromatin templates. Nature 413: 435-438, 2001.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002372-1	A00953	-	100 ug	$370		Add to Cart
BHA21002372-2	A00953-10ug	Sample	10 ug	$99		Add to Cart
BHA21002372-3	A00953-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002372-4	A00953-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002372-5	A00953-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002372-6	A00953-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002372-7	A00953-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002372-8	A00953-FITC	FITC	100 ug	$570		Add to Cart
BHA21002372-9	A00953-HRP	HRP	100 ug	$570		Add to Cart
BHA21002372-10	A00953-APC	APC	100 ug	$670		Add to Cart
BHA21002372-11	A00953-PE	PE	100 ug	$670		Add to Cart
BHA21002372-12	A00953-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002372-13	A00953-carrier-free	carrier-free	100 ug	$370		Add to Cart

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