Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205401

Mfr.No.	7124-MSM6-P0
Alternative Names	APC1 ; Cachectin ; Differentiation inducing factor (DIF) ; Macrophage cytotoxic factor (MCF) ; Necrosin ; TNF alpha ; TNF Macrophage Derived ; TNF Monocyte Derived ; TNF Superfamily Member 2 ; TNFA
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Extracellular(secreted)
Chromosome Location	6p21.33
Clonality	Monoclonal
Description	TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Host	Mouse
Immunogen	Recombinant N-terminal fragment of human TNF- (TNF706); Peptide corresponding to aa115-130 of human TNF- (P/T2)
Isotype	IgM
Positive Control	HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil or Histiocytoma
Reactivity	Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
Recombinant	FALSE
Regulatory	RUO

Swissprot	P01375
Uniprot	241570
Gene Id	7124

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, IHC-FP

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
IHC-FP	2-4μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205401	7124-MSM6-P0	20µg	$204.67		Add to Cart
BHA10205401	7124-MSM6-P0	100µg	$393.20		Add to Cart

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205401

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