Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205366

Mfr.No.	7124-MSM1-P1ABX
Alternative Names	APC1 ; Cachectin ; Differentiation inducing factor (DIF) ; Macrophage cytotoxic factor (MCF) ; Necrosin ; TNF alpha ; TNF Macrophage Derived ; TNF Monocyte Derived ; TNF Superfamily Member 2 ; TNFA
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Extracellular(secreted)
Chromosome Location	6p21.33
Clonality	Monoclonal
Description	Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Host	Mouse
Immunogen	A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF-, conjugated to thyroglobulin
Isotype	IgM Kappa
Positive Control	HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Reactivity	Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
Recombinant	FALSE
Regulatory	RUO

Swissprot	P01375
Uniprot	241570
Gene Id	7124

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-FP

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-FP	2-4μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205366	7124-MSM1-P1ABX	100µg	$393.20		Add to Cart

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205366

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