Human Anti-TIGIT neutralizing antibody recognizing the TIGIT binding region on immune cells.
assay conditions: Assays done according to protocols from TIGIT:CD155 homogenous assays (72029). CD155-his was mixed with various concentrations of the anti-TIGIT mAbs (0-100 nM) and TIGIT-biotin for 1h at RT. Then Strep donor (1:125) and Ni acceptor beads (1:250) were added for 1h. Plate read on the alphaLISA machine
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Note
Avoid freeze/thaw cycles.
Uniprot
Q495A1
Format
Aqueous buffer solution
Purification
Protein A affinity chromatography from HEK293 supernatants.
Shipping Temperature
-80°C (dry ice)
Storage Stability
Stable for at least 12 months at -80°C. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.