SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.
Host
Mouse
Immunogen
Recombinant human SOX2 protein fragment (aa176-305) (exact sequence is proprietary)
Isotype
IgG2b Kappa
Positive Control
MCF-7 cells or Cervical carcinoma
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P48431
Uniprot
518438
Gene Id
6657
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
ELISA, FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., ELISA ; FACS ; IF ; WB ; IHC-F
Dilution
ELISA
Use Ab at 2-4μg/ml for coating
FACS
0.5-1μg/million cells
IF
1-2μg/ml
WB
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 min at RT
Reviews of Anti-SOX2 (Transcription Factor) Antibody BHA10205004