Since chicken IgY molecules are quite different from mammalian IgG’s, our secondary antibodies don’t cross react with primary antibodies made in rabbits, mice, or any other mammalian host tested. This makes it simple to performed double immunostaining.
Host
Chicken
Immunogen
Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column , Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100µg/100ul
Description
Specificity: Antibodies were tested for specificity using double-immunodiffusion in agarose. There is no detectable cross-reactivity with goat, human or mouse IgG.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IF, ELISA
Description
1:1000 Western blots, 1:250 Immunohistochemistry., WB ; IF ; ELISA
Dilution
WB
1:250
Reviews of Anti-Rabbit IgG Unlabeled Antibody (Chicken Host)