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Home / Primary Antibodies

Anti-POLK (DNA polymerase kappa) Antibody

SKU : BHA10920072

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y052099
Categories	Primary Antibodies
Clonality	polyclonal
Description	POLK (also known as DNA polymerase kappa,DINB protein and DINP) is involved in DNA repair. POLKplays an important role in translesion synthesis, where thenormal high-fidelity DNA polymerases cannot proceed andDNA synthesis stalls. Depending on the context, it inserts thecorrect base, but causes frequent base transitions,transversions and frameshifts. POLK lacks 3'-5' proofreadingexonuclease activity. POLK is a nuclear protein that binds toREV1L and PCNA. POLK is detected throughout the nucleusand at replication foci. Multiple alternative splice variants havebeen detected for this protein. POLK is found at low levels intestis, spleen, prostate and ovary and at very low levels inkidney, colon, brain, heart, liver, lung, placenta, pancreas andperipheral blood leukocytes.
Formulation	Liquid
Host	Goat(Gt)
Guarantee	abm guarantees that all our Anti-POLK (DNA polymerase kappa) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
Isotype	IgG
Quantity	100 μg
Recommended Dilution	This affinity purified antibody has been tested for use in ELISA and western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band ~ 98.8 kDa in size corresponding to POLKby western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weightsof 89.0 and 76.4 kDa.
Shipping Condition	Ice Packs
Species Reactivity	Human
Specificity	This affinity purified antibody is directed against human POLK protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivitywith this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence.Cross reactivity with POLK protein from mouse is expected, as this sequence is highly homologous in human andmouse. Expect reactivity with multiple

Gene Name	POLK
Clone No	Polyclonal

Concentration	1µg/ul
Purity	Affinity Purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C

Description	ELISA ; IHC ; WB

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SKU	Supplier No.	Size	Your price	Quantity
BHA10920072	Y052099	100 ug	$1162.50		Add to Cart

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