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Anti-Plasma Kallikrein 1B (Fletcher factor, Plasma Kallikrein, PK), Carboxy end catalytic domain Antibody

SKU : BHA10924669

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y051626
Categories	Primary Antibodies
Clonality	polyclonal
Description	The antibody is a rabbit polyclonal antibody made to the serine protease plasma kallikrein. The antibody is made to a synthetic peptide based on the carboxy end of the catalytic domain of human plasma kallikrein. The antibody reacts with human, mouse and rat plasma Kallikrein. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.Plasma Kallikrein 1B, also known as Fletcher factor, is a serine proteinase of the S1 family, and is secreted by the liver, kidney, adrenal gland and placenta. The zymogen is referred to as prekallikrein (PK) and is activated by Factor XIIa to an active two-chain form, plasma kallikrein. In Fletcher syndrome plasma kallikrein is deficient, resulting in a prolonged partial thromboplastin time (PT). Plasma Kallikrein cleaves at P1 Arg and Lys, and is responsible for liberating bradykinin from kininogen. Factor XIIa cleavage at Arg371-Ile372 generates two chains, a heavy and light chain, which resolve on reduced SDS PAGE gels, but remain associated in solution. Normal plasma kallikrein concentrations range from 35-50 ug/ml. Plasma kallikrein is produced as a 638 amino acid protein with predicted mass of 71,370 Dalton and a pI of 9.30. The 19 amino acid signal sequence is followed by four "Apple" cystein-rich domains in the heavy chain. The catalytic domain is in the light chain, and contains the canonical His-Asp-Ser catalytic triad. Glycosylation and other post translational modifications give plasma kallikrein a higher apparent molecular weights of 88 and 85 kDa . An autocatalytic cleavage of plasma kallikrein at Lys140-Ala141 generates a form termed ? -Kallikrein, with reduced enzymatic activity. RP3-Plasma Kallikrein 1B recognizes the zymogen form of plasma kallikrein (PK) and the light chain of factor XIIa cleaved plasma Kallikrein. Plasma Kallikrein is largely complexed with its substrate, H-kininogen in plasma, and plasma Kallikrein released from H-kininogen can cleave Factor XII, prorenin, prourokinase, C-1 complement, as well as converting factor XIIa to a more active form, factor XIIf. Plasma Kallikrein is partially inhibited by ? -2 Macroglobulin and completely inhibited by C-1 inhibitor in-vivo. Recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Formulation	Liquid
Host	Rabbit(Rb)
Guarantee	abm guarantees that all our Anti-Plasma Kallikrein 1B (Fletcher factor, Plasma Kallikrein, PK), Carboxy end catalytic domain Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	A synthetic peptide based on the carboxy end of the catalytic domain of human Plasma Kallikrein 1B.
Isotype	IgG
Quantity	100μg
Recommended Dilution	Recommended dilutionsWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 71 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Relevance	Plasma Kallikrein 1B is a serine proteinase of the S1 family It is secreted by the liver, kidney, adrenal gland and placenta. The zymogen is referred to as prekallikrein (PK) and is activated by Factor XIIa to an active two-chain form, plasma kallikrein. In Fletcher syndrome plasma kallikrein is deficient, resulting in a prolonged partial thromboplastin time (PT).
Shipping Condition	Ice Packs
Specificity	This antibody is specific for Plasma Kallikrein 1B (carboxy end of catalytic domain)

Gene Name	Plasma Kallikrein 1B
Clone No	Polyclonal

Concentration	1µg/ul
Purity	Immunogen Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C

Description	WB

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SKU	Supplier No.	Size	Your price	Quantity
BHA10924669	Y051626	100 ug	$1357.50		Add to Cart

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