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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Pirh2/RCHY1 Antibody Picoband®

SKU : BHA21005054

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Mfr.No.	A04533-3, A04533-3-10ug, A04533-3-Biotin, A04533-3-Cy3, A04533-3-Dylight488, A04533-3-Dylight550, A04533-3-Dylight594, A04533-3-FITC, A04533-3-HRP, A04533-3-APC, A04533-3-PE, A04533-3-iFluor647, A04533-3-carrier-free
Alternative Names	RAB11B, member RAS oncogene family
Background	RING finger and CHY zinc finger domain-containing protein 1 is a protein that in humans is encoded by the RCHY1 gene. The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alteatively spliced transcript variants encoding different isoforms have been described for this gene.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Pirh2/RCHY1 Antibody Picoband® catalog # A04533-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to
Host	Rabbit
Immunogen Region	E.coli-derived human Pirh2/RCHY1 recombinant protein (Position: Q17-Q250).
Isotype	Rabbit IgG
Molecular Weight	21 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3
Tissue Specificity	Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q96PM5
Gene Id	25898
Gene Name	RCHY1
Subcellular Location	Recycling endosome membrane, Lipid-anchor, Synaptic vesicle membrane, Phagosome membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.Avoid repeated freezing and thawing.

Applications	WB, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 ug/ml, -<br>

Reference	1. Fedorova, O. , Gudovich, A. , Daks, A. , Baidyuk, E. , & Barlev, N. A. . (2021). Regulation of autophagy flux by e3 ubiquitin ligase pirh2 in lung cancer. Biochemical and Biophysical Research Communications, 563, 119-125.<br /><br />2. Yifeng, Bao, Xue, Wu, Debin, & Yuan, et al. (2017). High expression of pirh2 is associated with poor prognosis in glioma. Cellular and molecular neurobiology.<br /><br />3. Yang, ShuyunChen, YingyingSun, FulanNi, QichaoWang, HuaHuang, YeqingZhang, ChunhuiLiu, KunWang, ShuoQiu, JianXu, ZhenHua, LuHe, Zhixian. (2016). Downregulated pirh2 can decrease the proliferation of breast cancer cells. Archives of medical research, 47(3).

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21005054-1	A04533-3	-	100 ug	$370		Add to Cart
BHA21005054-2	A04533-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21005054-3	A04533-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21005054-4	A04533-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21005054-5	A04533-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21005054-6	A04533-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21005054-7	A04533-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21005054-8	A04533-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21005054-9	A04533-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21005054-10	A04533-3-APC	APC	100 ug	$670		Add to Cart
BHA21005054-11	A04533-3-PE	PE	100 ug	$670		Add to Cart
BHA21005054-12	A04533-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21005054-13	A04533-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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