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Home / Primary Antibodies / Monoclonal Antibodies

Anti-PGP9.5 Antibody Picoband® (monoclonal, 3E4)

SKU : BHA21003399

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Mfr.No.	M01018-6, M01018-6-10ug, M01018-6-Biotin, M01018-6-Cy3, M01018-6-Dylight488, M01018-6-Dylight550, M01018-6-Dylight594, M01018-6-FITC, M01018-6-HRP, M01018-6-APC, M01018-6-PE, M01018-6-iFluor647, M01018-6-carrier-free
Alternative Names	caseinolytic mitochondrial matrix peptidase chaperone subunit
Background	UCH-L1, also known as PGP9.5, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. The catalytic triad of UCH-L1 contains a cysteine at position 90, an aspartate at position 176, and a histidine at position 161 that are responsible for its hydrolase activity.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-PGP9.5 Antibody Picoband® (monoclonal, 3E4) catalog # M01018-6. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP (PubMed:28874591). Targets specific substrates for degradation by the Clp complex (PubMed:11923310, PubMed:22710082). Can perform chaperone functions in the absence of CLPP. E
Host	Mouse
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.
Isotype	Mouse IgG1
Molecular Weight	27 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; CLPX
Tissue Specificity	Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	P09936
Gene Id	7345
Gene Name	UCHL1
Clone No	Clone: 3E4
Subcellular Location	Mitochondrion; mitochondrion nucleoid

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, Flow Cytometry
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Rat<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. "Entrez Gene: UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)".<br /><br />2. Das C, Hoang QQ, Kreinbring CA, Luchansky SJ, Meray RK, Ray SS, Lansbury PT, Ringe D, Petsko GA (Mar 2006). "Structural basis for conformational plasticity of the Parkinson's disease-associated ubiquitin hydrolase UCH-L1". Proceedings of the National Academy of Sciences of the United States of America 103 (12): 467580.<br /><br />3. Doran JF, Jackson P, Kynoch PA, Thompson RJ (Jun 1983). "Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis". Joual of Neurochemistry 40 (6): 15427.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003399-1	M01018-6	-	100 ug	$370		Add to Cart
BHA21003399-2	M01018-6-10ug	Sample	10 ug	$99		Add to Cart
BHA21003399-3	M01018-6-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003399-4	M01018-6-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003399-5	M01018-6-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003399-6	M01018-6-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003399-7	M01018-6-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003399-8	M01018-6-FITC	FITC	100 ug	$570		Add to Cart
BHA21003399-9	M01018-6-HRP	HRP	100 ug	$570		Add to Cart
BHA21003399-10	M01018-6-APC	APC	100 ug	$670		Add to Cart
BHA21003399-11	M01018-6-PE	PE	100 ug	$670		Add to Cart
BHA21003399-12	M01018-6-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003399-13	M01018-6-carrier-free	carrier-free	100 ug	$370		Add to Cart

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