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Home / Primary Antibodies / Polyclonal Antibodies

Anti-PCNA Antibody Picoband®

SKU : BHA21002357

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Mfr.No.	A00125, A00125-10ug, A00125-Biotin, A00125-Cy3, A00125-Dylight488, A00125-Dylight550, A00125-Dylight594, A00125-FITC, A00125-HRP, A00125-APC, A00125-PE, A00125-iFluor647, A00125-carrier-free
Alternative Names	proliferating cell nuclear antigen
Background	Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase delta in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-PCNA Antibody Picoband® catalog # A00125. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and
Host	Rabbit
Immunogen Region	E.coli-derived human PCNA recombinant protein (Position: M1-S261).
Isotype	Rabbit IgG
Molecular Weight	36 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	P12004
Gene Id	5111
Gene Name	PCNA
Subcellular Location	Nucleus. Secreted.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, IF, IHC, ICC, WB, Flow Cytometry
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Rat, By Heat<br> Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Moldovan GL, Pfander B, Jentsch S (May 18, 2007). "PCNA, the maestro of the replication fork". Cell. 129 (4): 66579. 2. Warbrick E (Mar 1998). "PCNA binding through a conserved motif". BioEssays. 20 (3): 1959. 3. Mailand N, Gibbs-Seymour I, Bekker-Jensen S (May 2013). "Regulation of PCNA-protein interactions for genome stability". Nature Reviews. Molecular Cell Biology. 14 (5): 26982.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002357-1	A00125	-	100 ug	$370		Add to Cart
BHA21002357-2	A00125-10ug	Sample	10 ug	$99		Add to Cart
BHA21002357-3	A00125-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002357-4	A00125-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002357-5	A00125-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002357-6	A00125-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002357-7	A00125-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002357-8	A00125-FITC	FITC	100 ug	$570		Add to Cart
BHA21002357-9	A00125-HRP	HRP	100 ug	$570		Add to Cart
BHA21002357-10	A00125-APC	APC	100 ug	$670		Add to Cart
BHA21002357-11	A00125-PE	PE	100 ug	$670		Add to Cart
BHA21002357-12	A00125-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002357-13	A00125-carrier-free	carrier-free	100 ug	$370		Add to Cart

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