Specificity: This Polyclonal antibody is directed against human p53 protein. The product was purified from seurm by protein A chromatography. Expect reactivity with human p53. Cross-reactivity with p53 from other sources has not been determined.Relevance: p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (2). p53 is frequently mutated or inactivated in about 60% of cancers (3).
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
Recommended dilutionsWB: 1/2000.Predicted molecular weight: 53 kDa.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB