(39 kDa) transcription factor expressed in embryonic stem cells (ESC) that function with other factors such as NANOG and SOX2 to regulate pluripotency. Down regulation of OCT4 has been shown to trigger cell differentiation, thus it can be used as a marker for undifferentiated ESC or IPS (induced pluripotent stem) cells. ABM’s anti-NANOG Monoclonal Antibody is a proven product that is suitable for your research needs.
Host
Mouse
Immunogen
Recombinant human OCT4 protein is used to produce this monoclonal antibody
Isotype
IgG
Quantity
100 µl
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100µg/100ul
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IHC, ELISA
Description
The suggested dilutions are: Western Blot 1:500 to 1:1000, ELISA 1:5000, Immunohistochemistry 1:50 to 1:100, Immunofluorescence 1:10 to 1:50, Flow Cytometric 1:10 to 1:50., WB ; IHC ; ELISA