Anti-Notch1 Antibody

Mfr.No.	Y058066
Categories	Primary Antibodies
Cellular Localization	Cell Membrane
Clonality	polyclonal
Description	The Notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm).
Host	Rabbit
Immunogen	Synthetic peptide conjugated to KLH
Isotype	IgG
Quantity	100 µl
Reactivity	Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	PBS, pH 7.4 with 0.02% Sodium Azide
Concentration	100µg/100ul
Description	Specificity: Notch1 antibody detects endogenous levels of Notch1 protein. Not tested with other proteins Relevance: The Notch signaling pathway was first identified in Drosophila. The Notch 1 protein is a single pass transmembrane receptor activated through the Delta (Dl) and Serrate (Ser) ligands in Drosophila (and their mammalian counterparts Delta like 3 and Jagged 1 and 2). Activation results in the proteolytic cleavage of the cytoplasmic portion of Notch (120kDa) which localizes to the nucleus to interact with DNA binding proteins (RB Jk/KBF2/CBF1). The interaction of Notch with these proteins converts them from transcriptional repressors to activators, thus affecting the expression of a variety of target genes. During maturation, the Notch molecule is cleaved by furin like convertase at the extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C terminal Notch 1 fragment dissociates from its N terminal counterpart and is further cleaved between Gly1743 and Val1744. The resulting activated cytosolic fragment translocates to the nucleus and activates Notch related transcription.
Format	Liquid
Purity	Purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, ELISA

Description

Recommended dilutions Peptide ELISA: 1:160,000 WB: 1/500 - 1/1000 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; ELISA

Dilution

ELISA	1:160,000
WB	1:500-1:1000

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SKU	Supplier No.	Size	Your price	Quantity
BHA10916350	Y058066	100 ul	$217.50		Add to Cart

Anti-Notch1 Antibody

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