Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. NF-L was purified by ion exchange chromatography, followed by preparative gel electrophoresis.
Immunogen Region
Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. NF-L was purified by ion exchange chromatography, followed by preparative gel electrophoresis.
Raised In
Rabbit
Shipping Condition
Ice packs
Species Reactivity
Human,Mouse,Rat,Other
Specificity
NF-L from human, rat, mouse, cat, chicken.
Note
Immunofluorescence: 1:250 to 1:500.ABC or other enzyme linked immunocytochemical procedures: 1:2,500. Western blots: 1:10,000.
Concentration
100ug/100ul
Storage
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.