To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography , To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography
Quantity
100 µg
Reactivity
Rat, Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: NF-L from human, rat, mouse, cat, chicken
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IF
Description
The IgY solution can be used at dilutions of at least 1:2,500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5,000 or lower., WB ; IF
Reviews of Anti-NF-L (Chicken Polyclonal) Antibody