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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Nestin Antibody Picoband®

SKU : BHA21000775

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Mfr.No.	PB9874, PB9874-10ug, PB9874-Biotin, PB9874-Cy3, PB9874-Dylight488, PB9874-Dylight550, PB9874-Dylight594, PB9874-FITC, PB9874-HRP, PB9874-APC, PB9874-PE, PB9874-iFluor647, PB9874-carrier-free
Alternative Names	Nestin
Background	Nestin is a type VI intermediate filament (IF) protein that in humans is encoded by the NES gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α-internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Nestin Antibody Picoband® catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells duri
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
Isotype	Rabbit IgG
Molecular Weight	230 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Nestin;NES;Nbla00170;
Tissue Specificity	CNS stem cells.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P48681
Gene Id	10763
Gene Name	NES
Subcellular Location	Nucleus .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1ug/ml Western blot, 0.1-0.5ug/ml

Reference	1. Guérette D, Khan PA, Savard PE, Vincent M (2007). "Molecular evolution of type VI intermediate filament proteins". BMC Evol. Biol. 7: 164. 2. Michalczyk K, Ziman M (April 2005). "Nestin structure and predicted function in cellular cytoskeletal organisation". Histol. Histopathol. 20 (2): 66571.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000775-1	PB9874	100 ug	-	$370		Add to Cart
BHA21000775-2	PB9874-10ug	10 ug	Sample	$99		Add to Cart
BHA21000775-3	PB9874-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000775-4	PB9874-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000775-5	PB9874-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000775-6	PB9874-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000775-7	PB9874-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000775-8	PB9874-FITC	100 ug	FITC	$570		Add to Cart
BHA21000775-9	PB9874-HRP	100 ug	HRP	$570		Add to Cart
BHA21000775-10	PB9874-APC	100 ug	APC	$670		Add to Cart
BHA21000775-11	PB9874-PE	100 ug	PE	$670		Add to Cart
BHA21000775-12	PB9874-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000775-13	PB9874-carrier-free	100 ug	carrier-free	$370		Add to Cart

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