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Home / Primary Antibodies / Polyclonal Antibodies

Anti-NDUFB10 Antibody Picoband®

SKU : BHA21003072

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Mfr.No.	A11886-3, A11886-3-10ug, A11886-3-Biotin, A11886-3-Cy3, A11886-3-Dylight488, A11886-3-Dylight550, A11886-3-Dylight594, A11886-3-FITC, A11886-3-HRP, A11886-3-APC, A11886-3-PE, A11886-3-iFluor647, A11886-3-carrier-free
Alternative Names	solute carrier family 2 member 6
Background	NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 10 is an enzyme that in humans is encoded by the NDUFB10 gene. The protein encoded by this gene is an accessory subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) that is not ly involved in catalysis. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alteative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.(7) Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-NDUFB10 Antibody Picoband® catalog # A11886-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Facilitative glucose transporter; binds cytochalasin B with low affinity.
Host	Rabbit
Immunogen Region	E.coli-derived human NDUFB10 recombinant protein (Position: D36-S172).
Isotype	Rabbit IgG
Molecular Weight	22 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Solute carrier family 2, facilitated glucose transporter member 6; Glucose transporter type 6; GLUT-6; Glucose transporter type 9; GLUT-9; SLC2A6; GLUT9
Tissue Specificity	Highly expressed in brain, spleen and peripheral blood leukocytes.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	O96000
Gene Id	4716
Gene Name	NDUFB10
Subcellular Location	Cell membrane. Multi-pass membrane protein.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Emahazion, T., Beskow, A., Gyllensten, U., Brookes, A. J. Intron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain. Cytogenet. Cell Genet. 82: 115-119, 1998.<br /><br />2. Loeffen, J. L. C. M., Triepels, R. H., van den Heuvel, L. P., Schuelke, M., Buskens, C. A. F., Smeets, R. J. P., Trijbels, J. M. F., Smeitink, J. A. M. cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed. Biochem. Biophys. Res. Commun. 253: 415-422, 1998.<br /><br />3. Smeitink, J., van den Heuvel, L. Human mitochondrial complex I in health and disease. Am. J. Hum. Genet. 64: 1505-1510, 1999.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003072-1	A11886-3	-	100 ug	$370		Add to Cart
BHA21003072-2	A11886-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21003072-3	A11886-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003072-4	A11886-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003072-5	A11886-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003072-6	A11886-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003072-7	A11886-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003072-8	A11886-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21003072-9	A11886-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21003072-10	A11886-3-APC	APC	100 ug	$670		Add to Cart
BHA21003072-11	A11886-3-PE	PE	100 ug	$670		Add to Cart
BHA21003072-12	A11886-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003072-13	A11886-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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