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Home / Primary Antibodies

Anti-Myc Tag Antibody (Rabbit Polyclonal)

SKU : BHA10918326

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Applied Biological Materials (abm) Inc.

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Mfr.No.	G077
Categories	Primary Antibodies ; Tag & Cell Markers
Clonality	polyclonal
Description	Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve a universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Host	Rabbit
Immunogen	Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L
Isotype	IgG
Quantity	100 µg
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Concentration	1 mg/ml
Description	Specificity: This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Format	Liquid
Purity	Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, ELISA

Description

ELISA 1:1,000 ;
WESTERN BLOT 1:500 - 1:1,000 ;
IMMUNOHISTOCHEMISTRY User Optimized ;
OTHER APPLICATIONS User Optimized, WB ; ELISA

Dilution

ELISA	1:1,000
WB	1:500-1:1,000

Reference	DH, Seo et al. "The N-Terminal UND Motif of the Arabidopsis U-Box E3 Ligase PUB18 Is Critical for the Negative Regulation of ABA-Mediated Stomatal Movement and Determines Its Ubiquitination Specificity for Exocyst Subunit Exo70B1." The Plant Cell 12:2952-2973 (2016). DOI: 10.1105/tpc.16.00347. PubMed: 27956469. Application: Western Blot. Kim, JH et al. "The Arabidopsis RING E3 ubiquitin ligase AtAIRP3, LOG2 participates in positive regulation of high-salt and drought stress responses." Plant Physiol 162:1733-49 (2013). PubMed: 23696092.

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SKU	Supplier No.	Size	Your price	Quantity
BHA10918326	G077	100 ug	$330		Add to Cart

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