MAb TCR1 precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells. eglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates. Unlike human and mouse, in which γδ cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of γδ T cells. The frequency of TCRγδ cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.
Host
Mouse
Isotype
IgG1 Kappa
Quantity
100 µg
Reactivity
Avain
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/200ul
Description
Specificity: Chicken TCRγδ
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
FACS
Description
Immunoprecipitation IMMUNOFLUORESCENT STAINING: Chicken PBMC were double stained with mouse anti-chicken TCR γδ- FITC and mouse anti-chicken CD3-R-PE. Small lymphocytes then were gated and subjected to 2-color analysis on a FACScanâ„¢ flow cytometer, FACS
Reviews of Anti-Mouse Anti-Chicken TCR gammadelta-FITC Conjugate Antibody