Specificity: This Polyclonal antibody is directed against human MEK2 protein. The product was purified from seurm by protein A chromatography. Expect reactivity with human,mouse MEK2. Cross-reactivity with MEK2 from other sources has not been determined.Relevance: Microtubule associated protein kinase kinase 2 (MEK2, MAP2K2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
Recommended dilutionsWB: 1/500~3000.Predicted molecular weight: 44 kDa.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB