abm guarantees that all our Anti-MBP Tag Antibody (Maltose Binding Protein) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen
MBP epitope tag recombinant protein.
Isotype
IgG
Quantity
1.0 mg
Recommended Dilution
Optimal working dilutions should be determined experimentally by investigators. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 Immunoprecipitation: 5 µg/mg lysate Histo/Cytochemistry: 1:200
Shipping Condition
Ice Packs
Specificity
Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.
Gene Name
MBP
Concentration
100µg/100ul
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition
-20C
Description
IHC ; IP ; WB
Reviews of Anti-MBP Tag Antibody (Maltose Binding Protein)