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Home / Antibodies

Anti-MBP Tag Antibody (Maltose Binding Protein)

BHA10918495
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Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
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Buffer PBS, pH 7.4 with 0.05% sodium azide.
Concentration 100µg/100ul
Description Specificity: Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.
Format Liquid
Storage This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition -20C
Applications WB, IP, IHC
Description Optimal working dilutions should be determined experimentally by investigators. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 Immunoprecipitation: 5 µg/mg lysate Histo/Cytochemistry: 1:200, WB ; IP ; IHC
Dilution

WB1:1,000
IP1:200
Reference
  • Kim, WTK et al. "Suppression of Arabidopsis AtPUB30 resulted in increased tolerance to salt stress during germination." Plant Cell Reports 2:277-289 (2015). DOI: 10.​1007/​s00299-014-1706-4.
  • Mi, YB et al. "Homologous U-box E3 Ubiquitin Ligases OsPUB2 and OsPUB3 Are Involved in the Positive Regulation of Low Temperature Stress Response in Rice (Oryza sativa L.)." Front. Plant Sci. :16 (2017). DOI: 10.3389/fpls.2017.00016. Application: Western Blot.
  • Seo, DH et al. "The N-Terminal UND Motif of the Arabidopsis U-Box E3 Ligase PUB18 Is Critical for the Negative Regulation of ABA-Mediated Stomatal Movement and Determines Its Ubiquitination Specificity for Exocyst Subunit Exo70B1." The Plant Cell 12:2952-2973 (2016). DOI: 10.1105/tpc.16.00347. PubMed: 27956469. Application: Western Blot.
  • Trempe, FT et al. "Characterization of human herpesvirus 6A/B U94 as ATPase, helicase, exonuclease and DNA-binding proteins." Nucl. Acids Res : (2015). DOI: 10.1093/nar/gkv503. Application: WB.
SKU Supplier No. Size Your price Quantity
BHA10918495 G079 100 ug $232.50
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