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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Macrophage Antibody / S100A8 + S100A9 / Calprotectin

SKU : BHA17112828

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NSJ Bioreagents

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Mfr.No.	V2846, V2846-20UG, V2846-100UG
Clonality	Monoclonal
Description	Recognizes the macrophage L1 protein Calprotectin, expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	An affinity purified monocyte membrane preparation was used as the immunogen for the anti-Macrophage antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cytoplasmic

Antigen	Macrophage
Uniprot	P05109, P06702
Clone No	SPM281

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the anti-Macrophage antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	IHC-P
Dilution	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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