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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Ki67 Antibody (monoclonal, 5E12)

SKU : BHA21003394

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Mfr.No.	M00254-8, M00254-8-10ug, M00254-8-Biotin, M00254-8-Cy3, M00254-8-Dylight488, M00254-8-Dylight550, M00254-8-Dylight594, M00254-8-FITC, M00254-8-HRP, M00254-8-APC, M00254-8-PE, M00254-8-iFluor647, M00254-8-carrier-free
Alternative Names	tuberous sclerosis 2
Background	Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Ki67 Antibody Picoband® (monoclonal, 5E12) catalog # M00254-8. Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.
Function	In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400). Acts as a GTPase-activating protein (
Host	Mouse
Immunogen Region	E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
Isotype	Mouse IgG2b
Molecular Weight	35-60 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4
Tissue Specificity	Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	P46013
Gene Id	4288
Gene Name	MKI67
Clone No	Clone: 5E12
Subcellular Location	Cytoplasm; Membrane; Peripheral membrane protein

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, ICC, IF, Flow Cytometry
Description	Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Fonatsch, C., Duchrow, M., Rieder, H., Schluter, C., Gerdes, J. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991.<br /><br />2. Schonk, D. M., Kuijpers, H. J. H., vanDrunen, E., vanDalen, C. H., Geurts van Kessel, A. H. M., Verheijen, R., Ramaekers, F. C. S. Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum. Genet. 83: 297-299, 1989.<br /><br />3. Traut, W., Scholzen, T., Winking, H., Kubbutat, M. H. G., Gerdes, J. Assignment of the murine Ki-67 gene (Mki67) to chromosome band 7F3-F5 by in situ hybridization. Cytogenet. Cell Genet. 83: 12-13, 1998.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003394-1	M00254-8	-	100 ug	$370		Add to Cart
BHA21003394-2	M00254-8-10ug	Sample	10 ug	$99		Add to Cart
BHA21003394-3	M00254-8-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003394-4	M00254-8-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003394-5	M00254-8-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003394-6	M00254-8-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003394-7	M00254-8-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003394-8	M00254-8-FITC	FITC	100 ug	$570		Add to Cart
BHA21003394-9	M00254-8-HRP	HRP	100 ug	$570		Add to Cart
BHA21003394-10	M00254-8-APC	APC	100 ug	$670		Add to Cart
BHA21003394-11	M00254-8-PE	PE	100 ug	$670		Add to Cart
BHA21003394-12	M00254-8-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003394-13	M00254-8-carrier-free	carrier-free	100 ug	$370		Add to Cart

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