abm guarantees that all our Anti-Interleukin-4 (IL-4) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen
highly pure (>98%) recombinant hIL-4
Quantity
50 μg
Recommended Dilution
ELISA: To detect hIL-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection.IHC-P: Use at a concentration of 0.25 µg/ml.Neut: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required.WB:To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.
Shipping Condition
Ice Packs
Species Reactivity
Human
Specificity
hIL-4 100% reactivity
Gene Name
Interleukin-4 (IL-4)
Clone No
Affinity Purified Polyclonal Antibody
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer
PBS buffer, pH 7.4. Reconstitute in sterile waterat a concentration of >0.2 mg/ml.