G1m Marker ; G2m Marker ; G3m Marker ; G4m Marker ; HDC ; Heavy Chain Disease Protein ; Human Immunglobulin G ; Ig gamma1/2/3/4 Chain C Region ; IGHG1 ; IGHG2
Categories
Primary Antibodies
Cellular Localization
Cell Surface, Cytoplasmic (Secreted)
Chromosome Location
14q32.33
Clonality
Monoclonal
Description
This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of γ heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin�s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Host
Mouse
Immunogen
Purified human IgG
Isotype
IgG1, IgG2a Kappa
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01857; P01859; P01860; P01861
Uniprot
510635
Gene Id
3500;3501;3502;3503
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. NeoBiotechnologies is not liable for any property damage, personal injury, or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-IgG (Immunoglobulin Gamma Heavy Chain) Antibody BHA10202138