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Home / Antibodies

Anti-Human IL-6 Antibody

SKU : BHA10921803

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y050210
Categories	Primary Antibodies
Clonality	polyclonal
Formulation	Liquid
Host	Rabbit(Rb)
Guarantee	abm guarantees that all our Anti-Human IL-6 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	This antibody was prepared by repeated immunizations with recombinant human IL-6produced in E.coli.
Isotype	IgG
Quantity	100 μl
Recommended Dilution	This antibody has beentested for use in neutralizations, ELISA,immunohistochemistry, radioimmunoassay,immunoprecipitation and immunoblotting. Itrecognizes IL-6. Reactivity in otherimmunoassays is unknown. For immunoblotting,use the supernatants or lysates of 2 x 106endotoxin-stimulated human peripheral bloodmononuclear cells (PBMC). PBMC arestimulated for 24 hours with 1% (v/v) humanplus 10 ng/mL E.coli LPS. Forimmunohistochemistry, either paraffin fix orcryofix tissue. For immunoprecipitation, preclearingwith a non-specific rabbit IgG is helpfulto reduce background. For use in ELISAformats, this antibody is best used as thesecond antibody in combination with amonoclonal antibody as the capture antibody.
Shipping Condition	Ice Packs
Species Reactivity	Human
Specificity	This antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i.e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antibody is also useful for neutralization of human IL-6 activity in bioassays.For neutralization, incubate the sample with a 1:400 dilution of the antibody for at least 4 hours before testing. Acontrol of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments invitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as asoluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

Gene Name	Human IL-6
Clone No	Polyclonal

Concentration	1µg/ul
Purity	Purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C

Description	ELISA ; IHC ; WB

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SKU	Supplier No.	Size	Your price	Quantity
BHA10921803	Y050210	100 ul	$1162.50		Add to Cart

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