abm guarantees that all our Anti-Human IL-10 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-10produced in E.coli.
Isotype
IgG
Quantity
100 μl
Recommended Dilution
This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry,radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. The antibody recognizeshrIL-10. Reactivity in other immunoassays is unknown.
Shipping Condition
Ice Packs
Species Reactivity
Human
Specificity
This antiserum has been heated to 56°C for 30 minutes. The antiserum detectsrecombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiplebands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is alsouseful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbitIgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10.
Gene Name
Human IL-10
Clone No
Polyclonal
Concentration
1µg/ul
Purity
Purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.