Anti-HSP27 (Heat Shock Protein 27) Antibody BHA10201935

Mfr.No.	3315-MSM1-P1BX
Alternative Names	Heat shock 27kDa protein ; Estrogen-regulated 24kDa protein ; Heat shock 25kDa protein 1 ; Heat shock 28kDa protein 1 ; Heat shock protein beta-1 ; HMN2B ; Hsp25 ; Hsp28 ; HspB1 ; Stress-responsive protein 27 (SRP27)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Nuclear
Chromosome Location	7q11.23
Clonality	Monoclonal
Description	This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
Host	Mouse
Immunogen	Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Isotype	IgG1 Kappa
Positive Control	~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Reactivity	Human, Chimpanzee, Monkey, Sheep, Rat, Mouse, Chicken
Recombinant	FALSE
Regulatory	RUO

Swissprot	P04792
Uniprot	520973
Gene Id	3315

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
WB	0.25-0.5μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHA10201935	3315-MSM1-P1BX	100µg	$393.20		Add to Cart

Anti-HSP27 (Heat Shock Protein 27) Antibody BHA10201935

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Anti-HSP27 (Heat Shock Protein 27) Antibody BHA10201935

Rate it now..!!