Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
Formulation
Liquid
Host
Rabbit(Rb)
Guarantee
abm guarantees that all our Anti-Histone H4 tetra acetyl Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen
Peptide containing the amino terminal region of Tetrahymena histone variant hv1 acetylated at multiple lysines.
Isotype
IgG
Quantity
100μl
Recommended Dilution
The sequence of the immunogen fromTetrahymena hv1 has a high degree of homologyto histone H4 in higher organisms.This modification is conserved from yeast tohuman and a wide range of reactivity is expected.
Shipping Condition
Ice Packs
Species Reactivity
Human
Specificity
The immunogen has a high degree of homology to histone H4, so the antibody primarily recognizes tetra-acetylated histone H4, secondarily recognizes tri-acetylated forms of histone H4. Faint recognition of multiply acetylated histone H2A is observed.
Gene Name
Histone H4
Clone No
Polyclonal
Concentration
1µg/ul
Purity
Purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition
-20C
Description
ELISA ; WB
Reference
Shimizu, Met al. "Hydrolase controls cellular NAD, sirtuin, and secondary metabolites." Mol Cell Biol 32:3743-55 (2012).PubMed: 22801369.