abm guarantees that all our Anti-His Tag Antibody (Rabbit Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen
The antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
Isotype
IgG
Quantity
1.0 mg
Recommended Dilution
ELISA 1: 2500; WB 1:500-1000; IP 5 μg/sample; IF 5μg/mL; IHC 5 μg/mL
Shipping Condition
Ice Packs
Specificity
His-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
Gene Name
His
Concentration
100µg/100ul
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Storage Condition
-20C
Description
ELISA ; IHC ; IF ; IP ; WB
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