The antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
Isotype
IgG
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Concentration
100µg/100ul
Description
Specificity: His-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, IF, IHC, ELISA
Description
ELISA 1: 2500; WB 1:500-1000; IP 5 μg/sample; IF 5μg/mL; IHC 5 μg/mL, WB ; IP ; IF ; IHC ; ELISA
Dilution
ELISA
1:2500
WB
1:500-1000
Reviews of Anti-His Tag Antibody (Rabbit Polyclonal)