Depends upon the localization of the parent protein tagged with hexahistidine.
Clonality
monoclonal
Description
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Host
Mouse
Immunogen
6x His synthetic peptide.
Quantity
100 µg
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: His-tag fusion protein
Format
Liquid
Purity
IgG fraction
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, IF, IHC
Description
WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users., WB ; IP ; IF ; IHC
Dilution
WB
1:1,000-3,000
IS
1:500-2000
Reference
Bae, HJ et al. "DBC1 does not function as a negative regulator of SIRT1 in liver cancer." Oncol Lett 4 (5):873-877 (2012). PubMed: 23162614. Application: Western Blot.
Choi, J. W. et al. "Recombinant Human Laforin Expressed in Insect Cells: Expression, Purification, and Biochemical Characterizations." J. Korean Chem. Soc. 5:466-470 (2015). DOI: 10.5012/jkcs.2015.59.5.466. Application: WB.
Kim, S et al. "TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect ." Insect Biochem. Mol. Biol. 2017:31-42 (2017). DOI: 10.1016/j.ibmb.2017.08.007. Application: Western Blot.
Kwon, Y et al. "Biomimetic repeat protein derived from Xenopus tropicalis for fibrous scaffold fabrication." Biopolymers. 12:659-664 (2015). DOI: 10.1002/bip.22735. PubMed: 26297878.
Lim, KH et al. "Biotin-assisted folding of streptavidin on the yeast surface." Biotechnol prog 28 (1):276-283 (2012). DOI: 10.1002/btpr.721. PubMed: 21990063. Application: Immunoprecipitation.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI‐1 in regulation of cellular resistance of BCR‐ABL+ chronic myeloid leukemia cells to tyrosine." Proteomics 12 (13:2094-2106 (2012). DOI: 10.1002/pmic.201100553. Application: Western Blot.
Lone, S.A et al. "Molecular cloning and characterization of a novel vip3-type gene from Bacillus thuringiensis and evaluation of its toxicity against Helicoverpa armigera." Microbial Pathogenesis :464-469 (2018). DOI: 10.1016/j.micpath.2017.12.025. Application: Western Blot.
Mani, T et al. "FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function." Mol Cell Biol 31 (10):183-1996 (2011). DOI: 10.1128/MCB.00609-10. Application: Immunoblot.
Metzler, M et al. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1." J Neurosci 27:2298-308 (2007). PubMed: 17329427.
Mi, YB et al. "Homologous U-box E3 Ubiquitin Ligases OsPUB2 and OsPUB3 Are Involved in the Positive Regulation of Low Temperature Stress Response in Rice (Oryza sativa L.)." Front. Plant Sci. :16 (2017). DOI: 10.3389/fpls.2017.00016. Application: Western Blot.
Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev Biol 365 (2):353-75 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev Biol. 365:353-75 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
Olmstead, AD et al. "Human subtilase SKI-1, S1P is a master regulator of the HCV Lifecycle and a potential host cell target for developing indirect-acting antiviral agents." PLoS Pathog. 8:e1002468 (2012). PubMed: 22241994.
Paluzzi, JP et al. "The heterodimeric glycoprotein hormone, GPA2, GPB5, regulates ion transport across the hindgut of the adult mosquito, Aedes aegypti." PLoS ONE 9:e86386 (2014). PubMed: 24466069. Application: Western Blot.
Park, UH et al. "Piperine, a Component of Black Pepper, Inhibits Adipogenesis by Antagonizing PPARγ Activity in 3T3-L1 Cells." J Agric Food Chem 60 (15):3853-3860 (2012). DOI: 10.1021/jf204514a. PubMed: 22463744. Application: Western Blot.
Register, AC et al. "Allosteric Modulation of Src Family Kinases with ATP-Competitive Inhibitors." Kinase Signaling Networks :79-89 (2017). DOI: 10.1007/978-1-4939-7154-1_6. PubMed: 28730474 . Application: WB.
Takemae, H et al. "Toxoplasma gondii RON4 binds to heparan sulfate on the host cell surface." Parasitology International 2:123-130 (2018). DOI: 10.1013/j.parint.2017.10.008. Application: Western Blot.
Tayapiwatana, C et al. "Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors." BioMed Research International : (2015). DOI: http://dx.doi.org/10.1155/2015/853891.
Tsai, SL et al. "Functional display of complex cellulosomes on the yeast surface via adaptive assembly." ACS Synth Biol 2:14-21 (2013). PubMed: 23656322.
Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair." Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.
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Reviews of Anti-His Tag Antibody (Mouse Monoclonal)